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Construct size (kb): 5.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC
Intact vector size: 2.700
Type of vector: plasmid
Vector end: SmaI
Vector end: PstI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CBL
Other gene symbols: CBL2
Genomic copy number: unique
Gene name: Cas-Br-M (murine) ecotropic retroviral transforming
sequence
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 q23.3-qter
Tissue: HUT-78 and CCRF-CEM cell lines
Type of DNA: cDNA
Insert 5' end: ApaI
Insert 3' end: PstI
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: X57110
Nucleotides 1-2886 of the insert correspond to
nucleotides 1-2886 of X57110.

Insert lengths(kb): 2.900000095367432

Tissue: HUT-78 and CCRF-CEM cell lines

Gene product: Cas-Br-M (murine) ecotropic retroviral transforming sequence(Cas NS-1 viral (v-cbl) oncogene homolog) [CBL]

Restriction digests of the clone give the following sizes (kb): BglI--5.6; EcoRI--3.2, 2.4; PstI--2.9, 2.7; SacI--3.0, 1.5, 1.3.

The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.17, 1.5; EcoRI--0.58; HindIII--0.72, 1.62, 2.78; BglII--1.77.